Detection of Cork Taint Fault in Wine Using HS-SPME and GC-TOFMS for the Quantification of 2,4,6-trichloroanisole

DOCUMENT: Application | Ref: 203-821-516Download
Category: Aroma Flavour Fragrance, Separation Science Mass Spectrometry

The coupling of a HS-SPME sample preparation step together with the power of the Pegasus HT and the ChromaTOF deconvolution and calibration features, allowed the detection of very low concentration- at parts-per-trillion levels- of 2,4,6-trichloroanisole (TCA) in a real wine samples. This component, as well established, is responsible for the cork taint fault also at very low concentrations. Ref. 203-821-516

Cork taint is a classical fault in wine. The molecule responsible for this fault is the 2,4,6-trichloroanisole (TCA) that can develop in a natural cork and migrate into the wine. Very low amounts of TCA can render a wine undrinkable as the sensory threshold for most people is in the low parts per trillion (ppt) range. In this concern, a complete method was developed including an Headspace solid-phase micro-extraction (HS-SPME) sample preparation step coupled on-line with a GC-TOFMS analysis for the separation and detection of this analyte in wine samples. A commercially-available wine sealed with a screw cap was used as wine matrix, so naturally occurring TCA was not anticipated. Furthermore, 2,4,6-trichloroanisole (TCA) standard was used to spike real samples and to create a calibration curve to quantify this component. One benefit of LECO’s Pegasus HT that is particularly important in these analyses is the ability to handle matrix interferences and coelutions with ChromaTOF’s mathematical deconvolution tools.

The results demonstrate the ability to detect a target analyte namely the 2,4,6-tricholoranisole at parts-per-trillion levels within a real wine matrix that are comparable to the typical sensory thresholds. Calibration data were demonstrated and applied to a known spiked sample with good accuracy, even in the presence of a matrix coelution. Pegasus HT’s full mass range sensitivity and speed with unparalleled deconvolution capabilities allow you to get more out of your standard analyses without needing to run in selected ion monitoring mode to optimize quantitative results. Ref. 203-821-516